FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37233
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC610.16383315875701662No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC530.14234684285445706No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA520.13966105336663712No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT510.13697526387881717No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC500.13428947439099723No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT450.1208605269518975No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA440.11817473746407756No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG430.11548894797625761No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT430.11548894797625761No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA430.11548894797625761No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC410.11011736900061773No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC390.10474579002497784No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT390.10474579002497784No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC380.1020600005371579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGG204.277554E-4105.815529
GCGCATG356.842984E-493.91798145
CTGTACC250.001038457584.652412
CACGCCT250.001038457584.652419
TAAAAAG250.001038457584.652413
CCCACGC250.001038457584.652417
TCTGTAC250.001038457584.652411
CCTGGGG250.001038457584.652413
AGAGGCC250.001038457584.652418
CCACGCC250.001038457584.652418
CCCTGGG250.001038457584.652412
ACCCTGG300.002141250570.5436861
TGTACCC300.002141250570.5436863
CGCATGA500.00281930865.742584145
TCTTAGG350.003944654560.466012
GGCCCCA350.003944654560.466017
CTTAGGC350.003944654560.466013
CCACGAC502.2724282E-456.4349449
CTCTATA400.006691625352.907761
TCTATAC400.006691625352.907762
Produced by FastQC (version 0.11.5)