FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28532
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC440.15421281368288237No Hit
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC330.11565961026216177No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT320.11215477358755081No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG320.11215477358755081No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG320.11215477358755081No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA310.10864993691293987No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG310.10864993691293987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGAC100.008659577135.392973
GGACCAT100.008659577135.392976
CCATGTA100.008659577135.392979
ATGGACC100.008659577135.392974
CGCATTC100.008659577135.392978
GACCATG100.008659577135.392977
CATCAGC205.0268444E-4101.544742
CCATCAG205.0268444E-4101.544741
AGCGCCA258.7931845E-488.1988690-94
TTGGAAG259.339266E-486.87504685-89
CTTCCCT300.001378045478.74898120-124
TATACCG300.002515186867.696496
ATCAGCC300.002515186867.696493
TCAGCCT350.00463247658.0255624
TACCGCC350.00463247658.0255628
ATACCGC350.00463247658.0255627
CACTCTG400.00785664850.772375
GCCTACT650.0010114141.6593787
CCTACTC950.00650313628.5037868
TGCATCT350.004021339620.36360775-79
Produced by FastQC (version 0.11.5)