FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63692
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1650.2590592225083213No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG680.10676380079130818No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA640.10048357721534887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAT200.0070406506145.08578145
CGCATCA207.1854E-492.950949
ACTCGCA207.1854E-492.950946
TACTCGC207.1854E-492.950945
GTACGTG700.003520925362.179626145
ATGCTTT350.00661045953.1148266
CCATACA350.00661045953.1148262
CGGTGCC550.007141625552.05417130-134
TGGACAA700.002268932535.409885
GCCATAC1003.4615136E-430.9836481
AATGTTT850.00587117929.1610816
AGAGACC850.00587117929.1610814
AAATACA900.007761473327.5410214
AATACAG900.007761473327.5410215
ATACCCA1200.001005616725.8197086
GTTAGTC1250.001275927424.7869199
CATACAC1250.001275927424.7869193
GTATAGG1250.001275927424.7869191
ATAGGGG1601.9547119E-423.2377363
TTGGACA1400.002467171322.1311784
Produced by FastQC (version 0.11.5)