FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76435
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1500.19624517563943217No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1370.17923726041734808No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1250.1635376463661935No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1170.15307123699875713No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1140.14914633348596845No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1130.14783803231503892No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1050.13737162294760252No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT1030.13475502060574346No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT1020.1334467194348139No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT1010.13213841826388434No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1000.1308301170929548No Hit
TCCCTACCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCC990.12952181592202525No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT950.12428861123830706No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC950.12428861123830706No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC920.12036370772551841No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT920.12036370772551841No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC920.12036370772551841No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA870.11382220187087068No Hit
GCGGTGGGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGA830.1085889971871525No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC830.1085889971871525No Hit
GCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGTA810.10597239484529339No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA810.10597239484529339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)