FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76435
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1280.16746254987898215No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1220.15961274285340485No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1200.15699614051154576No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1120.1465297311441094No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1080.14129652646039118No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1050.13737162294760252No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1010.13213841826388434No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC980.12821351475109571No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT980.12821351475109571No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT940.12298031006737752No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT920.12036370772551841No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA900.11774710538365932No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG880.11513050304180021No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC840.10989729835808203No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG820.10728069601622293No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT790.1033557925034343No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG780.10204749133250475No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA770.10073919016157519No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC770.10073919016157519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG451.5514297E-597.077255145
ATTAGAG350.003887376760.7516143
GAGGCAC350.003887376760.7516147
TAGACCA553.5786178E-451.5468254
CCCATAG553.5786178E-451.5468251
CCATAGT605.496904E-447.251262
CCCTAGA658.154346E-443.6165472
AACCACG658.154346E-443.6165477
GTAAGAG658.154346E-443.6165479
CCACGAC658.154346E-443.6165479
AAGCCCG700.001174285240.5010765
CAAAATA1408.918074E-539.00425145
GGCACAT1200.001976470236.40397145
GACTACA800.00226285335.4384421
GACTGCT1001.5759339E-435.438447
CCCAGGG1052.0992522E-433.7508962
CCCCAGG1052.0992522E-433.7508961
AGGGTTG1551.7205075E-632.0089155
AGACTGC900.00403035931.5008376
ATCTCAT900.00403035931.5008377
Produced by FastQC (version 0.11.5)