FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN433.87038703870387No Hit
GTGTATTAGTTTATACTACAAGGACAGGCCCATTTGAGTATTTTGTTTTC30.27002700270027No Hit
CCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT20.18001800180018002No Hit
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT20.18001800180018002No Hit
CGGTAGTAGTTAGGATAATATAAATAGTTAAATTAAGAATGGTTATGTTA20.18001800180018002No Hit
CCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTAT20.18001800180018002No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA20.18001800180018002No Hit
ATCTTATAGAAATTTAGGTTAAATACAGACCAAGAGCCTTCAAAGCCCTC20.18001800180018002No Hit
GTAGTATTGGTTATGGTTCATTGTCCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGA20.18001800180018002No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAG20.18001800180018002No Hit
CGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTCAATTCCTCT20.18001800180018002No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC20.18001800180018002No Hit
GGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCT20.18001800180018002No Hit
GCCCATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGG20.18001800180018002No Hit
AATTAGAATCTTAGCTTTGGGTGCTAATGGTGGAGTTAAAGACTTTTTCT20.18001800180018002No Hit
GTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGAAG20.18001800180018002No Hit
ATTCTAACCTGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCAT20.18001800180018002No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA20.18001800180018002No Hit
GTTGTGAAGTATAGTACGGATGCTACTTGTCCAATGA20.18001800180018002No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGAT20.18001800180018002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)