FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN433.87038703870387No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC30.27002700270027No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCAT30.27002700270027No Hit
GCATTGCTGCGTGCTTGATGCTTGTCCCTTTTGATCGTGGTGATTTAGAG20.18001800180018002No Hit
ATCTGAGGGGGGTCATCCATGGGGACGAGAAGGGATTTGACTGTAATGTG20.18001800180018002No Hit
TACTTGCGCTGCATGTGCCATTAAGATATATAGGATTTAGCCTATAATTT20.18001800180018002No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT20.18001800180018002No Hit
TCATTGGACAAGTAGCATCCGTACTATACTTCACAAC20.18001800180018002No Hit
GCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTA20.18001800180018002No Hit
GTATAGGGGTTAGTCCTTGCTATGTTATGCTTGGTTATAATTTTTCATCT20.18001800180018002No Hit
TCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTAC20.18001800180018002No Hit
ATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCTAGTA20.18001800180018002No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT20.18001800180018002No Hit
ATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAG20.18001800180018002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTAT50.01519.0145
Produced by FastQC (version 0.11.5)