FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN796.491372226787181No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC30.24650780608052586No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG30.24650780608052586No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG20.16433853738701726No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG20.16433853738701726No Hit
GAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATG20.16433853738701726No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCC20.16433853738701726No Hit
ATATTGGCTAAGAGGGAGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTC20.16433853738701726No Hit
GTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTACCTCAGAAGT20.16433853738701726No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG20.16433853738701726No Hit
GGGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGG20.16433853738701726No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAAC20.16433853738701726No Hit
GATATATGGAGGATGGGGATTATTGCTAGGATGAGGATGGATAGTAATAG20.16433853738701726No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT20.16433853738701726No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAA20.16433853738701726No Hit
CTGTTAAAAGTGCATACCGCCAAAAGATAAAGTTTGAAATCTGGTTAGGCTGGTG20.16433853738701726No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG20.16433853738701726No Hit
GTTTATGTAGCTTACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGG20.16433853738701726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)