FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN796.491372226787181No Hit
ATATACACCAACAAACAATGTTCAACCAGTAACCACTACTAATC20.16433853738701726No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCC20.16433853738701726No Hit
TTTTAAAGGATAACAGCTATCCATTGGTCTTAGGCCCCAAAAATTTTGGT20.16433853738701726No Hit
CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA20.16433853738701726No Hit
ATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAG20.16433853738701726No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG20.16433853738701726No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG20.16433853738701726No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT20.16433853738701726No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC20.16433853738701726No Hit
GGCTCGTAGTGTTCTGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGC20.16433853738701726No Hit
GAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAATAACTT20.16433853738701726No Hit
CATCATAATCCTCTCTCAAGGACTTCAAACTCTACTC20.16433853738701726No Hit
GCCAATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTCTAACCTGA20.16433853738701726No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGA20.16433853738701726No Hit
GAATAATACTATCGCTGTTCATTATAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATAT20.16433853738701726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)