FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111516
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1780.1596183507299401No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1530.13720004304315075No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1480.13271638150579287No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT1470.13181964919832131No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1440.1291294522759066No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1410.12643925535349188No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1320.11836866458624772No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAGTCTTCTTACTCAT1170.10491767997417412No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1160.10402094766670253No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1160.10402094766670253No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT1150.10312421535923096No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1140.10222748305175938No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1130.10133075074428781No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA1120.10043401843681625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCC254.2018655E-8141.876134
CCCAGGC100.007558958141.876118
GTGACGG303.356587E-4112.57123145
CGAATCA400.001053364184.42842145
GTCATAA553.6241763E-581.86998145
GACCATA353.8143957E-581.0720751
CTCCAGT605.578946E-575.047485145
TCTTTGC502.6094822E-670.9380653
TGCCGCC502.6094822E-670.9380657
TATGCGA853.9121987E-666.21837145
CTATAAC350.003878787960.8040542
GCATTGC350.003878787960.8040546
CCGGTCT350.003878787960.8040549
ATTGCGG350.003878787960.8040548
GATAGCA350.003878787960.8040542
TCCGGTC350.003878787960.8040548
GCTATAC502.2300307E-456.7504543
GTAAAGC502.2300307E-456.7504543
AGTCTTT754.3365435E-756.750451
GATCACA400.005344663356.07261135-139
Produced by FastQC (version 0.11.5)