FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57609
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1050.18226318804353486No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1030.17879150827127707No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC970.16837646895450364No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC920.15969726952385913No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN890.1544897498654724No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT860.1492822302070857No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG760.13192383134579666No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT750.13018799145966775No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT740.12845215157353884No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC730.12671631168740996No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT710.12324463191515216No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT710.12324463191515216No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC690.11977295214289435No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC690.11977295214289435No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA640.11109375271224982No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC640.11109375271224982No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC640.11109375271224982No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG640.11109375271224982No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA630.10935791282612092No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT620.10762207293999201No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT620.10762207293999201No Hit
ACTATAACCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTACCAC590.1024145532816053No Hit
GTTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA580.1006787133954764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)