FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9813
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN790.8050545195149292No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT140.14266788953429124No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG130.13247732599612758No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA120.12228676245796392No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG120.12228676245796392No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT110.11209619891980026No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA110.11209619891980026No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC100.10190563538163662No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT100.10190563538163662No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG100.10190563538163662No Hit
CTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAGA100.10190563538163662No Hit
ATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTAGGCAGAT100.10190563538163662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)