FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9813
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN790.8050545195149292No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG140.14266788953429124No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC140.14266788953429124No Hit
CATAAATAGTATGGCTTTGAAGAAGGCGTGGGTACAGATGTGCAGGAATG120.12228676245796392No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG120.12228676245796392No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT110.11209619891980026No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC110.11209619891980026No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC110.11209619891980026No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA100.10190563538163662No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA100.10190563538163662No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG100.10190563538163662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)