FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12447
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250.20085161082991887No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA190.15264722423073832No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC180.14461315979754158No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG180.14461315979754158No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG170.13657909536434482No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA160.12854503093114808No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT160.12854503093114808No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT150.1205109664979513No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG150.1205109664979513No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT150.1205109664979513No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT140.11247690206475455No Hit
GGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTA130.10444283763155782No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA130.10444283763155782No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTCCAAGGACAA130.10444283763155782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)