FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12447
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250.20085161082991887No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC170.13657909536434482No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA170.13657909536434482No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT160.12854503093114808No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT160.12854503093114808No Hit
GTTGTATGTAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT160.12854503093114808No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA150.1205109664979513No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA140.11247690206475455No Hit
GTTGAAGAGGATAGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGA130.10444283763155782No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG130.10444283763155782No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT130.10444283763155782No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG130.10444283763155782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAGT100.0024809486204.75903145
CAAGAGC100.008192727137.611334
AGAGCCT100.008192727137.611336
CCAAGAG100.008192727137.611333
GACCAAG100.008192727137.611331
TATAGGG250.001128390482.56682
GTATAGG300.002326501368.805671
CTTCAAA200.007785723527.52226610-14
Produced by FastQC (version 0.11.5)