FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170.684931506849315No Hit
CCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTT40.16116035455278002No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGA40.16116035455278002No Hit
AGCATCAAGCACGCAACAATGCAGCTCAAAACGCTTAGCCTAGCCACACC40.16116035455278002No Hit
CTGCTAGGGCTGCAATAATGAAGGGTAAGATGAAGTGAAAGGTAAAGAAT40.16116035455278002No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA40.16116035455278002No Hit
ATAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTT30.12087026591458502No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT30.12087026591458502No Hit
GTTAGGGACGGATCGGAGAATTGTGTAGGCGAATAGGAAATATCATTCGG30.12087026591458502No Hit
GACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCT30.12087026591458502No Hit
ATCATATACCAAATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCT30.12087026591458502No Hit
ATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTC30.12087026591458502No Hit
GGAGAAGGCTTACGTTTAGTGAGGGAGAGATTTGGTATATGATTGAGATG30.12087026591458502No Hit
CGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGC30.12087026591458502No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA30.12087026591458502No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT30.12087026591458502No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG30.12087026591458502No Hit
AACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAG30.12087026591458502No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT30.12087026591458502No Hit
GTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTC30.12087026591458502No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA30.12087026591458502No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC30.12087026591458502No Hit
TCCTTGCCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTC30.12087026591458502No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT30.12087026591458502No Hit
GACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATA30.12087026591458502No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA30.12087026591458502No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG30.12087026591458502No Hit
GTGTTACATCGCGCCATCATTGGTATATGGTTAGTGTGTTGGTTAGTAGG30.12087026591458502No Hit
GTTAAATACAGACCAAGAGCCTTCAAAGCCCTCAGTAAGTTGCAATACTT30.12087026591458502No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG30.12087026591458502No Hit
ATATTACTGCTGTTAGAGAAATGAATGAACCTACAGATGATAGGATATTT30.12087026591458502No Hit
CATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCA30.12087026591458502No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC30.12087026591458502No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC30.12087026591458502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)