FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN160.6446414182111201No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC60.24174053182917005No Hit
GTGTATGGCTATCATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGG50.201450443190975No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC50.201450443190975No Hit
GGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGCAGT50.201450443190975No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA40.16116035455278002No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG40.16116035455278002No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAGGGGTAGCTTACTG40.16116035455278002No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA40.16116035455278002No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT40.16116035455278002No Hit
GGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTC40.16116035455278002No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA40.16116035455278002No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT40.16116035455278002No Hit
AGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCT40.16116035455278002No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT30.12087026591458502No Hit
CATAGGAGGTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCG30.12087026591458502No Hit
GTCTTTGTGGTTTGTAGAGAATAGTCAACGGTCGGCGAACATCAGTGGGG30.12087026591458502No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC30.12087026591458502No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC30.12087026591458502No Hit
ACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGAGGCTTA30.12087026591458502No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA30.12087026591458502No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT30.12087026591458502No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT30.12087026591458502No Hit
CTATAGGCGCTTGTTAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCA30.12087026591458502No Hit
GGGTAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGC30.12087026591458502No Hit
CGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCC30.12087026591458502No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA30.12087026591458502No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT30.12087026591458502No Hit
CCCTTCTCGTCCCCATGGATGACCCCCCTCAGATAGGAGTCCCTTGACCA30.12087026591458502No Hit
TTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTA30.12087026591458502No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC30.12087026591458502No Hit
GTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAACG30.12087026591458502No Hit
GTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTA30.12087026591458502No Hit
GAAGAGGATAGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGA30.12087026591458502No Hit
CCTTTAACCTCTCCACCCTTATCACAACACAAGAACACCTCTGATTACTC30.12087026591458502No Hit
CTTCTAGTAAGCCTCTACCTGCACGACAACACATAATGACCCACCAATCG30.12087026591458502No Hit
TTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAAC30.12087026591458502No Hit
GTAATATTGAACGTAGGTGCGATAAATAATGGGATGGGGTAGGAATCAAA30.12087026591458502No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC30.12087026591458502No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG30.12087026591458502No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC30.12087026591458502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTT100.009484832128.877557
ATGGCTA100.009484832128.877555
Produced by FastQC (version 0.11.5)