FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119028
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1950.163826998689384No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1550.13022146049668984No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1550.13022146049668984No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1530.12854118358705516No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1430.1201397990388816No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA1400.11761938367442955No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1350.11341869140034277No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1330.11173841449070807No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA1300.109217999126256No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1290.10837786067143865No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1240.10417716839735189No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT1200.10081661457808247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)