FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA130.19086771399207164No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG120.17618558214652769No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG120.17618558214652769No Hit
ATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCTA110.1615034503009837No Hit
CGGTACCATAAATACTTGACCACCTGTAGTACATAAAAACCCAATCCACA110.1615034503009837No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA110.1615034503009837No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC100.14682131845543972No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA100.14682131845543972No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT100.14682131845543972No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA100.14682131845543972No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC90.13213918660989576No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA90.13213918660989576No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT90.13213918660989576No Hit
ACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTACAGTCA90.13213918660989576No Hit
CCCCTATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAG90.13213918660989576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.13213918660989576No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA80.11745705476435178No Hit
ATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAG80.11745705476435178No Hit
GTACTATTTATACTAAAAGAGTAAGACCCTCATCAATAGATGGAGACATA80.11745705476435178No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA80.11745705476435178No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT80.11745705476435178No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA80.11745705476435178No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA80.11745705476435178No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC70.10277492291880781No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA70.10277492291880781No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT70.10277492291880781No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC70.10277492291880781No Hit
ATGATGGACCATGTAACGAACAATGCTACAGGGATGAATATTATGGAGAA70.10277492291880781No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT70.10277492291880781No Hit
TTATGGTACCGTACGATATTCATGGTGACTGGCAGTAATGTACGAAATAC70.10277492291880781No Hit
ATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG70.10277492291880781No Hit
ATTTATACTAAAAGAGTAAGACCCTCATCAATAGATGGAGACATACAGAA70.10277492291880781No Hit
GTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTACCCTTCATT70.10277492291880781No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG70.10277492291880781No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT70.10277492291880781No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG70.10277492291880781No Hit
TCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAA70.10277492291880781No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA70.10277492291880781No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA70.10277492291880781No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA70.10277492291880781No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC70.10277492291880781No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC70.10277492291880781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)