FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6811
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG170.24959624137424757No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG140.20554984583761562No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT130.19086771399207164No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA120.17618558214652769No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT110.1615034503009837No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC110.1615034503009837No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA110.1615034503009837No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT110.1615034503009837No Hit
GTATTTATGGTACCGTACGATATTCATGGTGACTGGCAGTAATGTACGAA100.14682131845543972No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT90.13213918660989576No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC90.13213918660989576No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA90.13213918660989576No Hit
GTTAAGGGTGGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTGGCTTT90.13213918660989576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.13213918660989576No Hit
GGTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAA90.13213918660989576No Hit
CTATAGGCGCTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCA80.11745705476435178No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA80.11745705476435178No Hit
TTATGGTACCGTACGATATTCATGGTGACTGGCAGTAATGTACGAAATAC80.11745705476435178No Hit
CTCGTAAGGGGTGGATTTTTCTATGTAGCCGTTGAGTTGTGGTAGTCAAA80.11745705476435178No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT80.11745705476435178No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA80.11745705476435178No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT80.11745705476435178No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT80.11745705476435178No Hit
CTATAGTTGTAGCAGGAGTCTTCTTACTCATCCGCTTCCACCCCCTAGCA70.10277492291880781No Hit
TTCTATGGCTGAGGGGAGTCAGGGGTGGAGACCTAATTGGGCTGATTTGC70.10277492291880781No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA70.10277492291880781No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG70.10277492291880781No Hit
CTCATAACCCTCAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGC70.10277492291880781No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT70.10277492291880781No Hit
GCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTTAGAAGGGCTATTTGTT70.10277492291880781No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC70.10277492291880781No Hit
ACATAAAGCCATTTACCGTACATAGCACATTACAGTCAAATCCCTTCTCG70.10277492291880781No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA70.10277492291880781No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC70.10277492291880781No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTA70.10277492291880781No Hit
CATTAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACC70.10277492291880781No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC70.10277492291880781No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC70.10277492291880781No Hit
ATATTCATGGTGACTGGCAGTAATGTACGAAATACATAGCGGTTGTTGAT70.10277492291880781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAA150.0067962217145.39464145
TACATCA150.0067962217145.39464145
TCTTGTT150.0067962217145.39464145
GGTGGGT100.0077856444139.51479
CTGTTAA100.0077856444139.51471
Produced by FastQC (version 0.11.5)