FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62629
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1220.19479793705791248No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1100.17563748423254402No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1030.16446055341774576No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1030.16446055341774576No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1020.16286384901563175No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT990.15807373580928963No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT960.1532836226029475No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC960.1532836226029475No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT850.1357198741796931No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC830.13252646537546506No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC810.12933305657123695No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA810.12933305657123695No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC790.1261396477670089No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT790.1261396477670089No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT760.12134953456066679No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT740.11815612575643872No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG740.11815612575643872No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN730.11655942135432468No Hit
ATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATG710.11336601255009661No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA700.11176930814798255No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC690.11017260374586854No Hit
GTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGGGGGTGGAAGCGG680.1085758993437545No Hit
GACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATGC670.10697919494164045No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC670.10697919494164045No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG660.10538249053952643No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG650.10378578613741238No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC650.10378578613741238No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC640.10218908173529834No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT640.10218908173529834No Hit
GCTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGT640.10218908173529834No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC630.10059237733318431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAG100.007610929141.5025
ATCCAGG100.007610929141.5026
CGCATGA709.926225E-997.63872145
CCTAACG400.001001576385.433876145
CCTGGGG703.6379788E-1180.8582843
CCCTGGG703.6379788E-1180.8582842
CATCGAC606.775372E-874.12856115-119
GCCCCAC300.00198520971.9589585-89
TTAGAGT300.002123262770.7514
GTTAGAG300.002123262770.7513
ACTGATA500.002428250968.34711145
CCACGAC755.7698344E-966.034269
TTAGAGG1257.481503E-963.79063145
ACCCTGG903.4197E-1062.889781
AAACCTC451.3373735E-462.889786
GGTTAGT1350.062.889788
CCTCTTG751.5792216E-460.75298145
GCGCGTA350.00391153460.6437159
ACAATGC350.00391153460.6437153
GCTACAG350.00391153460.6437158
Produced by FastQC (version 0.11.5)