FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62629
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1240.19799134586214054No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1190.19000782385157036No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1070.17084737102620193No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1020.16286384901563175No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT970.15488032700506157No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC960.1532836226029475No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT950.15168691820083346No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC930.1484935093966054No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC920.14689680499449137No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT890.14210669178814925No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA880.14050998738603523No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC870.1389132829839212No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC760.12134953456066679No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC720.11496271695221062No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN720.11496271695221062No Hit
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG710.11336601255009661No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA680.1085758993437545No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT680.1085758993437545No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG670.10697919494164045No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT670.10697919494164045No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG650.10378578613741238No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC630.10059237733318431No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG630.10059237733318431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTC100.0075918096141.621117
AACTGCC100.0075918096141.621115
TCCGTGC609.094947E-1294.414088
CCGTGCG651.8189894E-1187.151459
TAGTCCG801.8189894E-1279.661875
TGTAGTC851.8189894E-1274.9758763
GTAGTCC1103.092282E-1157.935914
GTCCGTG1008.6401997E-1056.6484387
GGGAGCC400.00661342553.1079147
AGGGAGC400.00661342553.1079146
GTATCCG650.007415615551.55728145
CATGATG700.00993897847.874622145
AGAGCGG605.5146223E-447.207045
AGTCCGT1352.3283064E-1047.2070356
AGGGTAC856.072585E-541.6532674
GTTGTAG1704.9112714E-1141.6532671
TTGTAGT1651.6661943E-938.6239362
GTTAGTC750.001653662837.765639
GGAGCCT951.16944924E-437.268718
GGTTAGT1355.861257E-736.7165838
Produced by FastQC (version 0.11.5)