FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175703
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA2790.15879068655629103No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC2610.1485461261333045No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2550.14513127265897566No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT2430.138301565710318No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2380.13545585448171063No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC2330.13261014325310325No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2160.12293472507583822No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT2160.12293472507583822No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC2120.1206581560929523No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT2040.11610501812718053No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1870.1064295999499155No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1830.10415303096702959No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1790.1018764619841437No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA1790.1018764619841437No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT1770.10073817749270074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG750.085.512139
CCCTGGG953.5739504E-852.507452
CGTTGAA650.00840987150.0031145
CCTGGGG1005.3536496E-849.8820763
TTAGAGT605.371884E-447.506744
TCCGTGC1352.237357E-1047.506748
AGCCTTA605.371884E-447.506746
AAGGAGC605.371884E-447.506746
CCATAGC752.8147819E-547.5067379
ATACCAT752.8147819E-547.5067376
TGTAGTC1403.2014214E-1045.810073
TCAGAGT956.552345E-445.616863145
AGCCCGT804.1211126E-544.537576
CATGTAC804.1211126E-544.537578
GCATGTA804.1211126E-544.537577
AAAGGAG1202.2454878E-741.5683985
CTTACCG908.255168E-539.5889479
CTCTCCG750.001610966638.005399
AACACAG1355.655111E-736.9496845
TTAGAGG3551.8189894E-1236.621983145
Produced by FastQC (version 0.11.5)