FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175703
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC2610.1485461261333045No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2400.13659413897315356No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA2380.13545585448171063No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT2340.1331792854988247No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2120.1206581560929523No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA2080.11838158711006642No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1910.10870616893280138No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1880.10699874219563694No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1880.10699874219563694No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1760.10016903524697927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCA651.0126166E-466.574295145
GTTATAG554.47652E-664.800162
CCGTGCG851.4830221E-858.7013249
TACATGC701.8687208E-550.9144172
TAAGACT701.8687208E-550.9144179
TCCGTGC1005.3418262E-849.8961268
CTAAGTG1102.2905928E-549.174194145
CACAATG605.3644285E-447.5201237
GTACATG752.8100998E-547.520121
TCAGAGA1152.9789673E-547.03619145
ATGGTCT952.1486994E-645.0190624
CCCTAGT657.958006E-443.864731
GGCTCCC657.958006E-443.864739
CACTACA1008.4960274E-443.273293145
ATATACA1351.1823431E-842.240111
TATGGTC1054.2768606E-640.7315333
TGTAGTC1253.086643E-739.9169043
TGGTCTG1808.54925E-1139.60015
GAGGCAC908.241479E-539.60017
AGCCTTA1105.887001E-638.8800966
Produced by FastQC (version 0.11.5)