FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT80.2104709287029729No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA80.2104709287029729No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT70.1841620626151013No Hit
CCCTTAATAGTACATAGCACATAAAGTCATTTACCGTACATAGCACATTA60.15785319652722968No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA60.15785319652722968No Hit
GTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAACG60.15785319652722968No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60.15785319652722968No Hit
GACATAGGGTGCTCCGGCTCCAGCGTCCCGCAATGCTATCGCGTGCATAC60.15785319652722968No Hit
GACCAAGAGCCTTCAAAGCCCTCAGTAAGTTGCAATACTTAATTTCTGTA60.15785319652722968No Hit
GGGTAGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTG50.13154433043935806No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG50.13154433043935806No Hit
GTACTGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGC50.13154433043935806No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG50.13154433043935806No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC50.13154433043935806No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT50.13154433043935806No Hit
CTTCAGTAAGTATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA50.13154433043935806No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT50.13154433043935806No Hit
ATATTGTACAGTACCATAAATACTTGACTACCTGTAGTACATAAAAACTC40.10523546435148645No Hit
CTCTTCAATCAGCCACATGGCCCTCGTAGTAACAGCCATTCTCATCCAAA40.10523546435148645No Hit
CCCATCAACAACCGCTATGTATTTCGTACATTACTGCCAGCCACCATGAA40.10523546435148645No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA40.10523546435148645No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC40.10523546435148645No Hit
GTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATACATAGCGGTTG40.10523546435148645No Hit
CCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAATAGTACTTGCCG40.10523546435148645No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC40.10523546435148645No Hit
GCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACC40.10523546435148645No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC40.10523546435148645No Hit
AACTGACACTGAGCCACAACCCAAACAACCCAGCTCTCCCTAAGCTTCAA40.10523546435148645No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGAGGCTCACTCAC40.10523546435148645No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG40.10523546435148645No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG40.10523546435148645No Hit
TCTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACTAT40.10523546435148645No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG40.10523546435148645No Hit
ATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCCACAGA40.10523546435148645No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC40.10523546435148645No Hit
CTATTAAGGGTGGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTGGCT40.10523546435148645No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA40.10523546435148645No Hit
GCTAAGGACTGCAAAACCTCACTCTGCATCAACTGAACGCAAATCAGCCA40.10523546435148645No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC40.10523546435148645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC100.007581976139.93422
CCTTGCT100.007581976139.93423
GCTATAT100.007581976139.93427
GTCCTTG100.007581976139.93421
CTTGCTA100.007581976139.93424
Produced by FastQC (version 0.11.5)