FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT100.26308866087871613No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC90.23677979479084454No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT80.2104709287029729No Hit
CTTCAGTAAGTATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA80.2104709287029729No Hit
ATATTGTACAGTACCATAAATACTTGACTACCTGTAGTACATAAAAACTC70.1841620626151013No Hit
CGCCTACACAATTCTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTG70.1841620626151013No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA60.15785319652722968No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG60.15785319652722968No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60.15785319652722968No Hit
CCCTTAACAGTACATAGCACATAAAGTCATTTACCGTACATAGCACATTA60.15785319652722968No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC60.15785319652722968No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC50.13154433043935806No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC50.13154433043935806No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA50.13154433043935806No Hit
ATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCTA50.13154433043935806No Hit
GTAAAGAATCGTGTGAGGGTGGGACTGTCTACTGAGTAGCCTCCTCAGAT50.13154433043935806No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA50.13154433043935806No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG50.13154433043935806No Hit
CCTGTAATATTGAACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATC50.13154433043935806No Hit
GTGTAAGCTAGTCATATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTG50.13154433043935806No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG50.13154433043935806No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG50.13154433043935806No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC40.10523546435148645No Hit
TCAAAACCCTGCCCCCATGCTTACAAGCAAGTACAGCAATCAACCTTCAA40.10523546435148645No Hit
GGGTGAGTGAGCCTCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG40.10523546435148645No Hit
GTAATATTGAACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAA40.10523546435148645No Hit
CCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTAATTTACACTCACAAC40.10523546435148645No Hit
TTACTACACAATCAAAGACACCCTCGGCTTACTTCTCTTCCTTCTCTCCT40.10523546435148645No Hit
CGATAAACCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCC40.10523546435148645No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC40.10523546435148645No Hit
GCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTC40.10523546435148645No Hit
GTACCATAAATACTTGACTACCTGTAGTACATAAAAACTCAACCCACATC40.10523546435148645No Hit
GGTGAGTAGTGCATGGCTAGGAATAGTCCTGTGGTGATTTGGAGGATCAG40.10523546435148645No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG40.10523546435148645No Hit
CTATTAAGGGTGGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTGGCT40.10523546435148645No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC40.10523546435148645No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG40.10523546435148645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)