FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26808
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4011.4958221426439868No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG270.10071620411817368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTTG550.002434006434.831125
CAGGGTT550.002434006434.831124
GAAGCCG350.005996702729.676073100-104
Produced by FastQC (version 0.11.5)