FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26808
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4011.4958221426439868No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG310.115637123246792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATT200.0023272252208.71075145
TTTTCTC150.0027413636198.07059140-144
GGTGCTA150.0054777246157.3458115-119
TTTCAGG200.009640579130.17526125-129
AAGACTT150.005237228756.307693130-134
GGCGCAG300.00986496547.9203031
ATTATAC502.6122772E-547.9203033
CCATAGC300.00986496547.9203039
TAATACT459.05338E-442.5958254
TTATACC500.001520078538.3362434
TATTATA550.002427158634.8511282
TTACCCT600.0037180131.9468694
AATCAAC752.8471343E-431.9468696
CTAATAC650.005500365529.4894183
Produced by FastQC (version 0.11.5)