FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7733
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG150.19397387818440448No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT110.1422475106685633No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG110.1422475106685633No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT100.129315918789603No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG100.129315918789603No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT100.129315918789603No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC90.1163843269106427No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG90.1163843269106427No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC90.1163843269106427No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT90.1163843269106427No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90.1163843269106427No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC90.1163843269106427No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA80.10345273503168241No Hit
ATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAG80.10345273503168241No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA80.10345273503168241No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA80.10345273503168241No Hit
CTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATT80.10345273503168241No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT80.10345273503168241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA100.008616053134.980514
ATAGGAC100.008616053134.980513
GGACATA100.008616053134.980516
CTAAGAC100.008616053134.980518
TAAGACT100.008616053134.980519
GTCCTGT100.008616053134.980516
CCCTGGG151.5741284E-4134.980512
AGTCCTG100.008616053134.980515
CATGCTA100.008616053134.980514
CCTGGGG151.5741284E-4134.980513
GGGTTAG204.9464055E-4101.235397
TGGGGTT204.9464055E-4101.235395
ACCCTGG204.9464055E-4101.235391
GTTAGTA250.001200673480.988319
GGGGTTA250.001200673480.988316
CCCCTGA250.001200673480.988311
GGTTAGT300.002475401267.490268
CTGGGGT350.00455962257.8487974
GCTTAGT200.00851521626.99610315-19
Produced by FastQC (version 0.11.5)