FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128142
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC2680.20914298200433892No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT2240.17480607451108926No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT2100.16388069485414616No Hit
CTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAA2070.16153954207051552No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA2010.1568572365032542No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA1700.1326653244057374No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG1680.13110455588331696No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1650.12876340309968629No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT1580.12330071327121474No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG1570.12252032901000454No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1550.12095956048758408No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA1490.11627725492032277No Hit
CCTCTAGCCTAGCCGTTTACTCAATTCTCTGATCAGGGTGAGCATCAAAC1490.11627725492032277No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGA1440.11237533361427167No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT1410.110034180830641No Hit
GGATTAGACTGAGCCGAATTGGTATATAGTTTAAACAAAACGAATGATTT1360.10613225952458992No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCACCCTTCT1300.10144995395732859No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA1290.10066956969611836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAG100.0075001353142.255571
TAAGCCA100.0075001353142.255574
ATCTAGG407.300363E-571.1277851
TATGCGA1207.046765E-964.427185145
CTGCTTG350.003839275860.966677
CTTACCG651.2186336E-554.713689
CCTCCTG400.006513025653.3458373
GCACCGA853.437086E-451.974873145
TGGGTTA701.8896224E-550.8055575
ATTAGAG1152.906745E-949.48023
GAGGCAC1152.906745E-949.48027
TTGTATA1204.2382453E-947.418522
ATGCTGT752.8415052E-547.418526
TATGCTG752.8415052E-547.418525
TGTATAT1151.6264676E-743.2951743
ATATAGC1151.6264676E-743.2951746
GCTGGTA855.94954E-541.8398748
CTGGTAA855.94954E-541.8398749
CTAATGC855.94954E-541.8398743
TATATCG700.001156985340.6444475
Produced by FastQC (version 0.11.5)