FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59247
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1150.19410265498675036No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT890.15021857646800682No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG790.13334008473002854No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA680.1147737438182524No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA680.1147737438182524No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT670.11308589464445458No Hit
TTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCA600.10127095042786977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGG457.157289E-492.896866145
GACGTAG300.003135126864.122466
GCTAGAG300.003135126864.122462
ACGTAGA300.003135126864.122467
AATAGAC300.003135126864.122462
AAACTGA450.00503515856.86922120-124
ACAGGGT350.00577227254.9621058
TAGAGTG550.00944872848.497704130-134
GAATAGA400.00978632848.0918431
CGTAGAC400.00978632848.0918438
CCTGGGG400.00978632848.0918433
CCCTGGG400.00978632848.0918432
AAGACGT901.524167E-435.6235855
AAGAACA1601.548505E-424.0459214
AGAACAG1450.002478101322.1111935
GGTTAGT1700.006226253718.8595458
ATACTAA1750.00736015818.3207021
Produced by FastQC (version 0.11.5)