FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39788
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6221.5632854126872424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAA255.459329E-499.40340485-89
TCGAGTG305.632891E-498.56455100-104
AGGCGTC457.0434756E-493.02987135-139
GATTGAT400.001053715484.14913115-119
TGGGCGA450.002034996271.30586110-114
TATACCG250.00420186159.515416
TACGCCT250.00420186159.515417
AGTTAAT250.00420186159.515411
AATACGC404.3030683E-449.596185
CTCAGGC300.00864346949.5961769
TAATACG300.00864346949.5961764
ATAATAC553.7785794E-545.0874373
TCTACAT500.001291494839.676942
TACATAT500.001291494839.676944
ACATATT500.001291494839.676945
CTCTACA550.00206271636.0699461
TAGAGGG750.00940750226.4512965
GTAATAT750.00940750226.4512961
TAGGCCT750.00940750226.4512964
CTATACT1201.245911E-424.7980884
Produced by FastQC (version 0.11.5)