FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3860
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT110.2849740932642487No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG110.2849740932642487No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT90.233160621761658No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA70.18134715025906736No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT70.18134715025906736No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC60.15544041450777202No Hit
CTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGT60.15544041450777202No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA60.15544041450777202No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT60.15544041450777202No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA60.15544041450777202No Hit
TTATGGTACCGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATAC60.15544041450777202No Hit
ATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTG50.1295336787564767No Hit
GTTAAGGATAGGTAGGTTTGTTGATATCCTAGTGGGTGAGGGGTGGCTTT50.1295336787564767No Hit
TCCTAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATA50.1295336787564767No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50.1295336787564767No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC50.1295336787564767No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA50.1295336787564767No Hit
GTTGATGGGTGAGTCAATACTTGGGTGGTACCCAAATCTGCTTCCCCATG50.1295336787564767No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC40.10362694300518134No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA40.10362694300518134No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA40.10362694300518134No Hit
GTGTTATGGGCCCGGAGCGAGGAGAGTAGCACTCTTGTGCGGGATATTGA40.10362694300518134No Hit
GTAGTAAGGTGGAGTGGGTTTGGGGCTAGGTTTAGCTCAGAGCGGTCAAG40.10362694300518134No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACGCCGC40.10362694300518134No Hit
GAGTAGGAGAGTGATATTTGATCAGGAGAATGTGGTTACTAGCACAGAGA40.10362694300518134No Hit
GGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTGGGGCTAGGTTTAG40.10362694300518134No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA40.10362694300518134No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG40.10362694300518134No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG40.10362694300518134No Hit
GTATAAACTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCC40.10362694300518134No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC40.10362694300518134No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC40.10362694300518134No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA40.10362694300518134No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG40.10362694300518134No Hit
GTTGTTGATGGGTGAGTCAATACTTGGGTGGTACCCAAATCTGCTTCCCC40.10362694300518134No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT40.10362694300518134No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT40.10362694300518134No Hit
CTGTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATAT40.10362694300518134No Hit
TCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACATTCACAGCC40.10362694300518134No Hit
GGGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATG40.10362694300518134No Hit
GGGTAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGC40.10362694300518134No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG40.10362694300518134No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT40.10362694300518134No Hit
GAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA40.10362694300518134No Hit
GTGTAAGTTGGGTGCTTTGTGTTAAGCTACACTCTGGTTCGTCCAAGTGC40.10362694300518134No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC40.10362694300518134No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA40.10362694300518134No Hit
CCTTAACAGTACATGGTACATAAAGCCATTTACCGTACATAGCACATTAC40.10362694300518134No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA40.10362694300518134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAATA251.9134447E-4125.80408145
GGTTGGT300.00249257366.714287
GTTGGTC300.00249257366.714288
Produced by FastQC (version 0.11.5)