FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45243
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT660.14587892049598833No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC620.13703777379926177No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA590.13040691377671684No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC580.12819662710253518No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT520.11493490705744534No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT510.11272462038326371No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA500.11051433370908206No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC500.11051433370908206No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC490.10830404703490043No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG490.10830404703490043No Hit
GTCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT480.10609376036071877No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC470.10388347368653714No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA470.10388347368653714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCTG100.0075941896141.563059
CCGTGCG204.2266236E-4106.1722959
GAGTATC250.001026107284.937839
TCCGTGC250.001026107284.937838
TGTAGTC300.002115812970.781533
GATACCA500.002787790765.96546145
AATCAGT451.3305292E-462.9169166
GCCTTTC350.00389784660.669887
GGCCAGG350.00389784660.669886
GGAAATC350.00389784660.669883
CTTTCTA350.00389784660.669889
GCCAGGG350.00389784660.669887
TCAGTGA350.00389784660.669888
CCAGGGG400.00661231153.0861478
GTCCGTG553.5867305E-451.477477
GAAATCA658.172919E-443.557864
CCCTATA1001.5782173E-435.3907622
GTCCTAG1451.0202202E-634.170391
TGGGTTG1153.5853995E-430.7745765
TGTTTCT200.00673783128.3816835-39
Produced by FastQC (version 0.11.5)