FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75245
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1450.19270383414180345No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1410.18738786630340887No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1340.17808492258621836No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1130.1501760914346468No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1070.14220213967705495No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1020.13555717987906174No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1000.13289919595986444No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGAGGCTCACTCAC1000.13289919595986444No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT950.1262542361618712No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA940.12492524420227256No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT910.12093826832347665No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC850.11296431656588478No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT830.11030633264668749No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT830.11030633264668749No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT810.10764834872749018No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT780.10366137284869427No Hit
GAGTAGGGCTGAGACTGGGGTGGGGCCTTCTATGGCTGAGGGGAGTCAGG780.10366137284869427No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC760.10100338892949698No Hit
GCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACT760.10100338892949698No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT760.10100338892949698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATGC204.0941822E-4107.09579
GTACGGA204.0941822E-4107.09576
AACCACG301.7155273E-595.196187
CCACGAC353.6864498E-581.5967259
CGCATGA554.116189E-579.74605145
ATAATAC407.1457034E-571.397133
AGTACGG300.002049672771.397135
TGCGAGT500.002828832765.7905145
CCGTGCG451.2802215E-463.464129
TCCGTGC451.2802215E-463.464128
TAGTCAA701.359105E-462.657604145
GTGTTAC350.003776145661.1975441
TACATCG350.003776145661.1975445
CATCGCG350.003776145661.1975447
ACATCGC350.003776145661.1975446
ATCGCGC350.003776145661.1975448
TCGCGCC350.003776145661.1975449
CACCAGC350.003776145661.1975442
ACATCGA600.005823469754.82541145
TACTTCG605.30168E-447.598099
Produced by FastQC (version 0.11.5)