FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33133
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN590.17807020191350015No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC580.17505206289801709No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA510.1539250897896357No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC470.1418525337277035No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT430.1297799776657713No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA410.12374369963480518No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT400.12072556061932212No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA390.11770742160383907No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA390.11770742160383907No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC380.11468928258835602No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC370.11167114357287296No Hit
CCCCTGTACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG340.1026167265264238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)