FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33133
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC600.18108834092898318No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570.17203392388253402No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA480.14487067274318655No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT460.13883439471222045No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC430.1297799776657713No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA400.12072556061932212No Hit
ACATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA400.12072556061932212No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA390.11770742160383907No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG360.10865300455738991No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC360.10865300455738991No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT350.10563486554190686No Hit
GTACAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT350.10563486554190686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGA400.0012794380.20421145
ATACTCA350.00399047960.2746736
TATACTC350.00399047960.2746735
TCAAAAA550.004522781458.33033145
GGATTGC400.00676926552.7403376
CTGTGAC400.00676926552.7403379
ACAGGTC658.40692E-443.2741248
GTATAGG908.7698994E-539.0669171
TACTATT1000.00695409628.1281812
TATAGGG1000.00695409628.1281812
Produced by FastQC (version 0.11.5)