FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19936
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGT6113.0648073836276084No Hit
ATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATA5732.8741974317817016No Hit
TACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATG5292.653491171749599No Hit
GTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGA4282.1468699839486356No Hit
GTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGC2941.4747191011235954No Hit
GGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTA2881.4446227929373996No Hit
CCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACA2831.4195425361155698No Hit
GGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCG2701.3543338683788122No Hit
ATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAG2241.1235955056179776No Hit
CCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATC2131.0684189406099518No Hit
ATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGG2061.03330658105939No Hit
GGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCG1850.9279695024077047No Hit
GCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACA1790.8978731942215089No Hit
GCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATAC1680.8426966292134831No Hit
CCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTT1620.8126003210272873No Hit
CATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCA1450.7273274478330658No Hit
ATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAAT1340.6721508828250402No Hit
CAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTG1220.6119582664526484No Hit
GGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATA1110.5567817014446228No Hit
CCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTA1100.5517656500802568No Hit
CATTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGC1030.516653290529695No Hit
CATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTA1020.5116372391653291No Hit
GTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGC970.4865569823434992No Hit
ATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAAT890.4464285714285714No Hit
GATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAA880.44141252006420545No Hit
ACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGC860.4313804173354735No Hit
ATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTG850.4263643659711076No Hit
TGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATG720.3611556982343499No Hit
CATTTATTCTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATAC720.3611556982343499No Hit
ACATTGGTATCCGCCATCCCATACATTTATTCTTGGCATCTACGGCTCAA710.35613964686998395No Hit
CTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCT700.35112359550561795No Hit
ATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGG660.3310593900481541No Hit
GTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCC650.3260433386837881No Hit
GAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTA600.3009630818619583No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN600.3009630818619583No Hit
GCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGA590.2959470304975923No Hit
TCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATAC550.2758828250401284No Hit
GGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCA490.24578651685393257No Hit
TACCAATGTTGAGCCGTAGATGCCAAGAATAAATGTATGGGATGGCGGAT400.20064205457463882No Hit
AATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATG370.18559390048154095No Hit
CCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCA360.18057784911717495No Hit
ATACATTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATT360.18057784911717495No Hit
TGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGG360.18057784911717495No Hit
GAATAAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAAT330.16552969502407705No Hit
ACATTGGTATCCGCCATCCCATACATTTGGCATCTACGGCTCAACATTGG330.16552969502407705No Hit
ATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATG300.15048154093097915No Hit
CCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACAT280.1404494382022472No Hit
TTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATC280.1404494382022472No Hit
CATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATT270.1354333868378812No Hit
GTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAAGAATAAATG270.1354333868378812No Hit
ATACATTTATTCTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCA270.1354333868378812No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA260.13041733547351525No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT260.13041733547351525No Hit
ATGCCAAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAA260.13041733547351525No Hit
CCGCCATCCCATACATTTATTCTTGGCATCTACGGCTCAACATTGGTATC260.13041733547351525No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG250.12540128410914927No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA250.12540128410914927No Hit
CCAAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGT230.11536918138041735No Hit
TACCAATGTTGAGCCGTAGATGCCAAATGTATGGGATGGCGGATACCAAT220.11035313001605136No Hit
TCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGC220.11035313001605136No Hit
ATGTTGAGCCGTAGATGCCAAGAATAAATGTATGGGATGGCGGATACCAA210.10533707865168539No Hit
CCGCCATCCCATACATTTGGCATCTACGGCTCAACATTGGTATCCGCCAT210.10533707865168539No Hit
GATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAA210.10533707865168539No Hit
CCCATACATTTATTCTTGGCATCTACGGCTCAACATTGGTATCCGCCATC200.10032102728731941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)