FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19936
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGT5742.8792134831460676No Hit
ATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATA5082.5481540930979136No Hit
GTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGA4962.4879614767255216No Hit
TACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATG4862.437800963081862No Hit
GTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGC3461.735553772070626No Hit
CCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACA2891.4496388443017656No Hit
ATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAG2511.2590288924558588No Hit
ATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGG2131.0684189406099518No Hit
GGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTA2111.0583868378812198No Hit
GGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCG2081.043338683788122No Hit
CCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATC2021.013242375601926No Hit
GGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCG1830.9179373996789727No Hit
CCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTT1750.877808988764045No Hit
GCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACA1650.8276484751203852No Hit
GCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATAC1550.7774879614767255No Hit
CATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCA1550.7774879614767255No Hit
ATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAAT1300.6520866773675762No Hit
CCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTA1190.5969101123595505No Hit
CAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTG1060.531701444622793No Hit
ATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAAT1040.521669341894061No Hit
GTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGC1030.516653290529695No Hit
GGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATA1010.506621187800963No Hit
CATTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGC1010.506621187800963No Hit
ATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTG860.4313804173354735No Hit
ACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGC820.4113162118780096No Hit
TGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATG790.3962680577849117No Hit
GATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAA770.38623595505617975No Hit
CATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTA760.3812199036918138No Hit
CATTTATTCTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATAC670.3360754414125201No Hit
GCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGA660.3310593900481541No Hit
ATCTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGG640.32102728731942215No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN600.3009630818619583No Hit
GTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCC590.2959470304975923No Hit
GAGCCGTAGATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTA580.29093097913322635No Hit
TCCCATACATTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATAC580.29093097913322635No Hit
CTACGGCTCAACATTGGTATCCGCCATCCCATACATTGGCATCTACGGCT530.2658507223113965No Hit
ACATTGGTATCCGCCATCCCATACATTTATTCTTGGCATCTACGGCTCAA500.25080256821829855No Hit
GGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATGGCGGATACCA490.24578651685393257No Hit
GAATAAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAAT420.21067415730337077No Hit
TGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGG380.1906099518459069No Hit
ATACATTTATTCTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCA340.17054574638844303No Hit
TTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTATC320.16051364365971107No Hit
TACCAATGTTGAGCCGTAGATGCCAAGAATAAATGTATGGGATGGCGGAT320.16051364365971107No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT310.1554975922953451No Hit
ATACATTTGGCATCTACGGCTCAACATTGGTATCCGCCATCCCATACATT310.1554975922953451No Hit
AATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATG270.1354333868378812No Hit
ACATTGGTATCCGCCATCCCATACATTTGGCATCTACGGCTCAACATTGG260.13041733547351525No Hit
CCAATGTTGAGCCGTAGATGCCAAGAATAAATGTATGGGATGGCGGATAC250.12540128410914927No Hit
GATGCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAA250.12540128410914927No Hit
CCGCCATCCCATACATTTATTCTTGGCATCTACGGCTCAACATTGGTATC240.1203852327447833No Hit
GGTATCCGCCATCCCATACATTTATTCTTGGCATCTACGGCTCAACATTG220.11035313001605136No Hit
ATGCCAAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAA220.11035313001605136No Hit
TACCAATGTTGAGCCGTAGATGCCAAATGTATGGGATGGCGGATACCAAT220.11035313001605136No Hit
ATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGTATGGGATG210.10533707865168539No Hit
GCCAATGTATGGGATGGCGGATACCAATGTTGAGCCGTAGATGCCAATGT210.10533707865168539No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT200.10032102728731941No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA200.10032102728731941No Hit
ATGTTGAGCCGTAGATGCCAAATGTATGGGATGGCGGATACCAATGTTGA200.10032102728731941No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT200.10032102728731941No Hit
ATTGGTATCCGCCATCCCATACATTGGCATCTACGGCTCAACATTGGTAT200.10032102728731941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGTCA150.0058196224154.2453145
GCTTAGC100.008854601134.286448
CATTTGG1000.067.143211
ATTTGGC1000.067.143212
CCCTAAA400.00807983450.357412
TTTGGCA1451.4551915E-1146.3056683
TGGTATC13000.012.395675
GGTATCC13000.012.395676
GTATCCG13350.012.070697
TATCCGC13350.012.070698
TTGGTAT13400.012.0256514
ATTGGTA13600.011.84880353
CATTGGT13850.011.6349262
ACCAATG8751.231092E-811.5102652
ATCCGCC14250.011.3083319
GTTGAGC8056.4316373E-710.8430038
TTGAGCC8056.4316373E-710.8430039
TACCAAT8751.5734986E-710.7429151
TGTTGAG8351.0312342E-610.4534357
AATGTTG8351.0312342E-610.4534355
Produced by FastQC (version 0.11.5)