FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89263
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1430.16020075507209033No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1370.15347904506906557No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1210.1355544850609995No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1140.12771249005747062No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1090.12211106505494998No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAGTCTTCTTACTCAT1080.12099078005444586No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1070.11987049505394172No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1060.1187502100534376No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1040.11650964005242934No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1030.11538935505192521No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGCTTTTTGGAAAGTCATGTCA1010.11314878505091697No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC980.10978793004940456No Hit
GCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA910.10194593504587568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAT250.001034441284.833749
CGGTATA250.001034441284.833742
TTCGAGA250.001034441284.833748
CAGTTCG300.002132957370.6947865
GTTCGAG300.002132957370.6947867
GTGTCAG300.002132957370.6947861
AGTTCGA300.002132957370.6947866
GACTGTC300.002132957370.6947867
CCTGGGG451.3457747E-462.839813
CCCTGGG451.3457747E-462.839812
GGGACTG350.003929354360.595535
AAGTTGG350.003929354360.595535
ATCCCGT607.844243E-658.9123236
CTGTCTA400.00666563353.021099
ACTGTCT400.00666563353.021098
GATTATC400.00666563353.021092
ACCCTGG658.265807E-443.5044821
TTAGAGG1705.892498E-638.80799145
TATAGGA750.001670827337.7038885
GGGTGGC850.00308765233.2681357
Produced by FastQC (version 0.11.5)