FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89263
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1480.16580218007461098No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1260.14115591006352016No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1250.14003562506301603No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1190.13331391505999127No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1130.12659220505696647No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1090.12211106505494998No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1050.11762992505293347No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1040.11650964005242934No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1030.11538935505192521No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC1010.11314878505091697No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC950.10642707504789219No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA900.10082565004537154No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT900.10082565004537154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTC250.001031384684.896978
CAATTCG250.001031384684.896979
CACATCC353.861391E-580.854269
CGAATCA450.00208560671.06616145
CAACCGT350.00391777760.6406949
GGGACAG350.00391777760.6406945
GATGTAG400.006646023653.0606043
CAGTCTG400.006646023653.0606049
CCCATAG400.006646023653.0606041
TAAACCA605.5517093E-447.1649865
TGTATGG1101.190765E-745.0211222
CATAAGC804.283469E-544.217172
GCATAAG658.2355394E-443.536911
CTGCGTC658.2355394E-443.536919
ATAAGCC658.2355394E-443.536913
TACCAAT1009.099652E-442.639694145
CCCAGGG856.125656E-541.616162
CCCCAGG856.125656E-541.616161
CTGTGAC1202.3589018E-741.2693639
CGCATGA1050.001156870340.609238145
Produced by FastQC (version 0.11.5)