FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8515
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210.24662360540223136No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG140.1644157369348209No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA120.14092777451556077No Hit
CTAATACACCAGTCTTGTAAACCGGAGATGAAAACCTTTTTCCAAGGACA120.14092777451556077No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC120.14092777451556077No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT100.11743981209630064No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG100.11743981209630064No Hit
CTTTAGTAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA90.10569583088667059No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT90.10569583088667059No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA90.10569583088667059No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA90.10569583088667059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCC100.00938794131.232134
TCTAGGT100.00938794131.232134
GACAGCT100.00938794131.232137
CTAGGTA100.00938794131.232135
GAGAGGA100.00938794131.232137
AGGTAAC100.00938794131.232137
GTAACGA100.00938794131.232139
CTTCTAG100.00938794131.232132
GGTAACG100.00938794131.232138
TAGGTAA100.00938794131.232136
ATCATAT300.002776748765.616071
Produced by FastQC (version 0.11.5)