FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8515
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210.24662360540223136No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT160.18790369935408102No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA120.14092777451556077No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA110.1291837933059307No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110.1291837933059307No Hit
CCCTTAACAGTACATAGTACATAAAGCCATTTACCGTACATAGCACATTG110.1291837933059307No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA110.1291837933059307No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG100.11743981209630064No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG100.11743981209630064No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT100.11743981209630064No Hit
TTATGGTACCGTACAATATTCATGGTGGCTGGCAGTAATGTACGAAATAC100.11743981209630064No Hit
CTTTAGTAGGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAA90.10569583088667059No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA90.10569583088667059No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT90.10569583088667059No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)