FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65498
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5930.9053711563711869No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG860.13130171913646216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA150.0047764215165.18924125-129
GTCTAGG1150.004766589557.480526145
TTTGGCG400.00616875954.02955275-79
CCCCCCC354.643832E-543.62786140-144
ACACCAG753.646861E-641.6276936
CCAGTCT600.002509998934.6897439
GTATTAT650.003715604532.02131
ACTACAC650.003715604532.02133
TAATACA700.005339482329.7340662
ACTTACA700.005339482329.7340662
ACCAGTC700.005339482329.7340668
TTACACT750.00747932327.7517954
AATACAC750.00747932327.7517953
ATACACC1009.6210925E-426.0173074
CCCTAGG1350.005500495419.272088
Produced by FastQC (version 0.11.5)