FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72000
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1320.18333333333333332No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA990.13749999999999998No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT990.13749999999999998No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA890.1236111111111111No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC850.11805555555555555No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT840.11666666666666668No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG780.10833333333333332No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC770.10694444444444445No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC770.10694444444444445No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA740.10277777777777779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)