FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50154
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN910.18144116122343182No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG880.17545958447980223No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT850.16947800773617258No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT810.16150257207799976No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT770.15352713641982693No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA750.14953941859074052No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC750.14953941859074052No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC710.1415639829325677No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA650.12960082944530846No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA620.12361925270167884No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA580.115643817043506No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG580.115643817043506No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG570.1136499581289628No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC540.10766838138533316No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT540.10766838138533316No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT540.10766838138533316No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT520.10368066355624676No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT520.10368066355624676No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG510.10168680464170356No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT510.10168680464170356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGA255.0099145E-5181.01259145
TGATGTA753.8867474E-580.45004145
TAATCTC250.001724811974.53465
GTTAGTC250.001724811974.53469
AGGTCGA453.0479223E-669.013529
CAATCAG951.2536126E-463.51319145
GCGAGAA601.2654188E-463.5051135-139
TCCGTGC601.6776958E-562.267365130-134
GAGGTCG505.6960544E-662.1121648
TTATGGA800.005103856356.566437145
CTCTAAC452.5410517E-455.2108151
CCGTGCG601.6776958E-551.7601369
CTAACAC504.2747153E-449.689733
TTGGTCA504.2747153E-449.689739
CCCTACC504.2747153E-449.689739
TAGTCCG652.6934707E-547.7785875
GTTGGTC556.838761E-445.1724858
CAAAATA1357.1080256E-444.694466145
TACGCCT704.1729887E-544.365837
TGAGTGA759.2216546E-442.48832100-104
Produced by FastQC (version 0.11.5)