FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115607
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1820.15742991341354762No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1790.15483491484079684No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1630.14099492245279266No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1550.13407492625879056No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1500.12974992863753926No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1360.1176399352980356No Hit
CTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAA1330.11504493672528479No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1280.1107199391040335No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT1270.10985493957978323No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1260.10898994005553296No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT1210.10466494243428168No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1180.10206994386153087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTTG502.6920012E-586.93675145
AAGCGAG353.6628837E-581.749
TCACCGG500.002937574265.20256145
GGATGCC350.003755114561.3049967
GATGCCC350.003755114561.3049968
CAAAGAT550.00428516759.27506145
AATCCAG502.1417541E-457.2180025
GTAATCC502.1417541E-457.2180023
ATCCAGG502.1417541E-457.2180026
GCTATAC553.429358E-452.016373
GGGAAGC553.429358E-452.016376
AACCACG605.2678806E-447.6816677
CGGATAC752.7483984E-547.6816673
TAGGGAA605.2678806E-447.6816674
CTCTAGC1352.1464075E-1047.6816672
GGTAACC657.814964E-444.0138472
TCCTGTA657.814964E-444.0138477
CTCTGGG657.814964E-444.0138478
GTAACCT657.814964E-444.0138473
CCTCTGG657.814964E-444.0138477
Produced by FastQC (version 0.11.5)