FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115607
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1840.15915991246204814No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1780.15396991531654658No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1570.13580492530729107No Hit
CTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAA1450.12542493101628793No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1440.12455993149203769No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1430.12369493196778743No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1360.1176399352980356No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1350.11677493577378532No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT1240.10725994100703246No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1190.10293494338578113No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA1190.10293494338578113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAA100.007364228143.122451
GGGCTCG204.0625673E-4107.3418354
CCTTCTG502.4779565E-671.561239
ATTGTGG407.081036E-571.5612268
TATGCTG502.136032E-457.2489855
CACCGCA502.136032E-457.2489859
TACATGC400.006356830253.6709182
GCATTAC400.006356830253.6709186
GCGGGTA400.006356830253.6709189
ACACCTT553.4202065E-452.044536
ATGCTGT553.4202065E-452.044536
ACCTTGA553.4202065E-452.044538
CTCTAGC1352.1282176E-1047.7074852
TTAGAGT605.25384E-447.707484
GCTGAGC605.25384E-447.707489
ATATACA605.25384E-447.707481
TCTAGCA952.085022E-645.1965683
GGTATGC657.7941583E-444.0376783
GCATGGC657.7941583E-444.0376783
TACAGGT855.736645E-542.094847
Produced by FastQC (version 0.11.5)