FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45634
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC740.16215979313669632No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT630.138054959021782No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT540.11833282201867028No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT510.11175877635096639No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC500.10956742779506508No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC490.10737607923916377No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT490.10737607923916377No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC480.10518473068326246No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA480.10518473068326246No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA480.10518473068326246No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA470.10299338212736119No Hit
GGCTAGGAGGGTGTCGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT460.10080203357145988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGGC100.007666342141.117327
CTGATAC100.007666342141.117323
CCTGATA100.007666342141.117322
CCCCGGT301.8114544E-594.0782247
CCCCCGG301.8114544E-594.0782246
GTATACC301.8114544E-594.0782241
CCGGTCG301.8114544E-594.0782249
CCCGGTC301.8114544E-594.0782248
AAAAGTA353.8923645E-580.638475
AACTGTT450.001770526873.96494145
CTGCAAA350.0039470660.4788559
TGCACAC350.0039470660.4788555
TAAAAGT651.26493305E-554.2758984
TTAAAAG651.26493305E-554.2758983
TATATCT400.00669567752.9189955
GTTAAAA553.643337E-451.315392
GTCCTAA658.3015946E-443.420721
ATATAGC658.3015946E-443.420726
GTACTAT1054.5120723E-640.3192371
TATACTC750.001678076237.6312875
Produced by FastQC (version 0.11.5)