FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7408
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT130.17548596112311016No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120.16198704103671707No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110.148488120950324No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG100.13498920086393087No Hit
ATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCA100.13498920086393087No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT90.12149028077753779No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC90.12149028077753779No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA90.12149028077753779No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT80.10799136069114472No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG80.10799136069114472No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT80.10799136069114472No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA80.10799136069114472No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT80.10799136069114472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATGA100.0015079534240.2716145
GCAATAT150.0050740745160.18106145
ACGCATC100.009308426131.56
CATCCTT100.009308426131.59
GCATCCT100.009308426131.58
Produced by FastQC (version 0.11.5)